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In-vitro Analysis of Antimicrobial Activities of Green Synthesized Silver Oxide Nanoparticles on some Microorganisms found in Open Wound

Rahama Orahachi Abdulmaleek(1), Idongesit Edem Okon(2), George Iloegbu Ndukwe(3), Patricia Adamma(4), E. Ekwumemgbo(5),


(1) Ahmadu Bello University
(2) Nigerian Institute of Transport Technology
(3) Ahmadu Bello University
(4) Ahmadu Bello University
(5) Ahmadu Bello University
Corresponding Author

Abstract


The main barrier to open wound healing is biofilm formation on the wound by a group of microbes encapsulated in a self-produced extracellular polymeric substance with high resistance to many conventional antimicrobial therapies. There is a need for unique anti-biofilm strategies and antimicrobial agents. This study aimed to investigate the antimicrobial activities of green synthesized silver oxide nanoparticles (Ag2O-NPs) on some bacteria (Staphylococcus aureus, Bacillus subtilis, Escherichia coli, and Pseudomonas aeroginosa) and fungi (Candida albican) commonly found in open wound (In-vitro). Ag2O-NPs exhibited considerable zone of inhibition (ZOI) against the test microorganisms. The minimum inhibitory concentration of silver nanoparticles against the bacteria was 3.75 and 15 mg/mL against the bacteria and fungi, respectively. The minimum bactericidal concentration was 7.50 and 15.00 mg/mL for bacteria and fungi, respectively. Green synthesized Ag2O-NPs exhibited some antimicrobial activities, which can aid in wound healing.

Keywords


Antimicrobial activities; Green synthesized Ag2O-NPs; Inhibition zone; Minimum bactericidal concentration; Minimum inhibitory concentration; Wound

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